Survival of Density Subpopulations of Rabbit Platelets: Use of 51Cr- or “In-Labeled Platelets to Measure Survival of Least Dense and Most Dense Platelets Concurrently
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چکیده
The origin of the density heterogeneity of platelets was studied by measuring the survival of density subpopulations of rabbit platelets separated by discontinuous Stractan density gradient centrifugation. When a total population of 5tCr-labeled platelets was injected into recipient rabbits. the relative specific radioactivity of the most dense platelets decreased rapidly. In contrast. that of the least dense platelets had not changed 24 hr after injection. and then decreased slowly. To distinguish between the possibilities that most dense platelets are cleared from the circulation more quickly than least dense platelets or that platelets decrease in density as they age in the circulation. the concurrent survival of least dense and most dense platelets. labeled with either 5tCr or 111ln. was determined. Mean survival times of 5tCror 111ln-labeled total platelet populations. determined concurrently in the same rabbits. were identical, calculated from 1 hr values as 100%. However. the 1-hr recovery of tt1ln-labeled platelets was
منابع مشابه
Survival of density subpopulations of rabbit platelets: use of 51Cr-or 111In-labeled platelets to measure survival of least dense and most dense platelets concurrently.
The origin of the density heterogeneity of platelets was studied by measuring the survival of density subpopulations of rabbit platelets separated by discontinuous Stractan density gradient centrifugation. When a total population of 51Cr-labeled platelets was injected into recipient rabbits, the relative specific radioactivity of the most dense platelets decreased rapidly. In contrast, that of ...
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